REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial strains
Coxiella burnetii Nine Mile phase II (clone 4, RSA 439)	Hoover et al.56	N/A
Coxiella burnetii Nine Mile phase II Δdot/icm mutant	This study	N/A
Experimental models: Cell lines
African green monkey kidney (Vero) fibroblasts (CCL-81)	American Type Culture Collection	https://www.atcc.org/products/ccl-81
Deposited data
Subtomogram average of the T4SS of Coxiella Burnetii at pH 4.75	This study	EMD-28281
Subtomogram average of the T4SS of Coxiella burnetii at pH 7	This study	EMD-28282
Subtomogram average of the T4SS of Coxiella burnetii at pH7 with an inner membrane mask	This study	EMD-28283
Software and algorithms
UCSF Tomography	Zheng et al.58	https://emcore.ucsf.edu/ucsf-software
Eman2	Chen et al.60,62	https://blake.bcm.edu/emanwiki/EMAN2
UCSF ChimeraX	Goddard et al.66	https://www.rbvi.ucsf.edu/chimerax/
ImageJ	Schindelin et al.61	https://imagej.net/
IMOD	Kremer et al.59	https://bio3d.colorado.edu/imod/
Knossos	https://knossos.app
Blender	https://www.blender.org/