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. 2023 Mar 17;7(14):3485–3500. doi: 10.1182/bloodadvances.2022008345

Figure 3.

Figure 3.

The MPC complex is required for bioenergetic capacity of MM cells. (A) OCR monitored by the Seahorse XF96 extracellular flux analyzer in JJN3 and U266 cells (n = 5). (B) Analysis of the different mitochondrial metabolic parameters obtained from the OCR in panel A. Significance was determined using two-way ANOVA followed by a Dunnett test. ∗P ≤ .05; ∗∗P ≤ .005; ∗∗∗P < .0001. (C) Quantification of basal ECAR and stressed ECAR in JJN3 and U266 cells (n = 5). Significance was determined using two-way ANOVA followed by a Dunnett test. ∗P ≤ .05; ∗∗P ≤ .005. (D) Media metabolite analysis of JJN3 (n = 4) and U266 cells (n = 5), with a focus on extracellular glucose and lactate. Significance was determined using two-way ANOVA followed by a Dunnett test. ∗P ≤ .05; ∗∗P ≤ .005; ∗∗∗P < .0001. (E) The metabolic capacity and flexibility of cells were represented by plotting the basal, oligomycin-treated, and maximal rates of ATP production from glycolysis (JATP gly) and oxidative phosphorylation (JATP ox), upon MPC1 knockout in both JJN3 and U266 cells (n = 5). (F) Fold change in the bioenergetic capacity and of cells described in panel A (n = 5). Significance was determined using two-way ANOVA followed by a Dunnett test. ∗P ≤ .05. OCR, oxygen consumption rate.