Fig. 3.

Overview of in-silico engineering of IsPETase (a) is the structure of IsPETase from 5xjh.pdb [44]. The catalytic triads are shown in the ball-stick model, α helices are shown in shaded yellow, β strands are shown in blue, and the residue spots considered in various studies for mutagenesis are marked in magenta. (b) shows the sequence of IsPETase. $\alpha$ helices and $\beta$ strands are marked in yellow and blue. Catalytic triads (S160, H237, D206) are marked with stars, and red arrows point to the residue spots considered for mutation. (Rx) denotes the references as R1-[32], R2-[49], R3-[50], R4-[51], R5-[52], R6-[53], R7-[54], R8-[44].