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. 2023 Jun 29;26(7):107235. doi: 10.1016/j.isci.2023.107235

Figure 1.

Figure 1

Irregular band patterns of H3K9bhb antibodies

(A) Structures of BHB and structurally similar butyrate.

(B) Western blots of HEK293T cells and iMEFs treated with either BHB, butyrate, or TSA for 24 h. Butyrate and TSA are known deacetylation inhibitors. Ponceau S staining was used to confirm the equivalent loading of proteins. mono: monoclonal, poly: polyclonal. Data are representative of at least 3 independent experiments.

(C) Upper: schematic of experimental workflow. HEK293T cells were treated with 5 mM BHB or 5 mM butyrate. At 24 h after the treatment, cellular metabolites were extracted and subjected to untargeted metabolomics by LC-MS/MS. unTx indicates untreated HEK293T cells. Lower: relative intensities of BHB in the metabolome of each treatment condition. N = 5-6 technical replicates per treatment condition. Data are represented as mean ± SD and analyzed by 1-way ANOVA (p < 0.0001).