Figure 1.
Anatomical analysis of T. crocea and distribution of Symbiodiniaceae cells
(A) Underwater photograph of T. crocea found at – 1 m in Ishigaki, Okinawa.
(B) Dissected specimen (shell length 80.0 mm). 1; mantle (outer epidermal layer: EL), 2; mantle (inner tissue layer: IL), 3; kidney, 4; muscle (adductor muscle and posterior byssal/pedal retractor muscles), 5; ctenidia (gill), 6; gonads and digestive diverticula (DD; inside).
(C–H) Fluorescence micrographs of visceral parts of T. crocea under blue (460–490 nm) excitation light. (C) Mantle EL, (D) Mantle IL, (E) kidney, (F) muscle, (G) gonad, (H) digestive diverticula. The red dots in fluorescence micrographs (C, D, and H) indicate symbiont algal chlorophyll autofluorescence.