Figure 4.
Mass imaging data for cryosections of T. crocea
(A) Atlas of the HE-stained cryo-visceral-section recovered after MS imaging. M, muscle; K, kidney; G, gonad; and DD, digestive diverticula. Heatmaps (0–100%) show relative intensities for two representative ion species (16:0-22:6) and (16:0-18:1) of PC and DGCC, and lyso-PC (16:0), and lyso-DGCC (16:0) in positive mode, (M + Na)+ for PC and (M + H)+ for DGCC.
(B) HE-stained cryo-mantle-section and heatmaps.
(C) MS imaging data for the detection of GCC.
(D) LC‒MS analysis of GCC in each organ. Each histogram indicates the average value from five independent specimens ±SD. Detailed results of multiple comparisons are given in Figure S14.