Fig. 3.

Extracted ion UHPLC-HRMS chromatograms for protonated molecules (± 5 ppm), of BTX-2 substrate (A) and phase I metabolites with elemental change of +H₂(B), +H₄(C), +O (D), +H₂O (E), +H₄O (F), +O₂(G), +H₂O₂(H), +CH₆O (I), +H₄O₂(J), and +CH₄O₂(K). Data were obtained from aliquots taken after 60 min incubation with liver microsomes from human (HLM; blue) or L. campechanus (RSN1; red, vertically and horizontally offset) microsomes, except for m/z 915.5100, for which the fish data (red trace) were taken from incubation with A. probatocephalus (SHP) microsomes. Normalization levels (NL) (arbitrary units) for the chromatograms are indicated in the panel for each species and were constrained to a minimum value of 5.00E3 to reduce noise. Peaks are labelled with the compound ID numbers shown in Table 1.