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. 2023 Jun 16;26(7):107157. doi: 10.1016/j.isci.2023.107157

Figure 2.

Figure 2

DIRAS3 inhibits migration of NSCLC cells and downregulates the expression of RAC1

(A) Overexpression of DIRAS3 in H1792 and Calu-1 cells. Cell lysates were analyzed by western blotting with antibodies against DIRAS3 and ACTB.

(B) Transwell assays of H1792 and Calu-1 cells that overexpressed DIRAS3. The statistics were performed using two-tailed Student’s t-tests, ∗∗∗p < 0.001.

(C) Wound-healing scratch assays of Calu-1 cells that overexpressed DIRAS3. Quantification of the data from three independent experiments using the ImageJ software (∗∗p < 0.01, ∗∗∗p < 0.001; p-values were obtained using two-tailed Student’s t-tests).

(D) TRITC-phalloidin staining of H1792 and Calu-1 cells that overexpressed DIRAS3. The white arrows denote the typical protrusions.

(E) Overexpression of DIRAS3 in H1792, Calu-1, and 293FT cells. Cell lysates were analyzed by western blotting with antibodies against DIRAS3, RAC1, and ACTB.