REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-DIRAS3	Thermo Fisher Scientific	Cat# PA5-25785; RRID: AB_2543285
Anti-RNF19B	Sigma-Aldrich	Cat# HPA049587; RRID: AB_2680826
Anti-RNF19B	Sigma-Aldrich	Cat# SAB1301675
Anti-RAC1	Proteintech Group	Cat# 66122-1-Ig; RRID: AB_2881521
Anti-RAC1	Cell Signaling Technology	Cat# 2465S
Anti-Flag	Sigma-Aldrich	Cat# F7425; RRID: AB_439687
Anti-His	Sigma-Aldrich	Cat# SAB1306085
Anti-Myc	Sigma-Aldrich	Cat# C3956; RRID: AB_439680
Anti-V5	MBL Life Science	Cat# M215-3
Anti-HA	Sigma-Aldrich	Cat# H3663
Anti-ACTB	Sigma-Aldrich	Cat# A1978; RRID: AB_476692
Anti-GAPDH	Sigma-Aldrich	Cat# G8795; RRID: AB_1078991
Biological samples
Fetal bovine serum	Sigma-Aldrich	F0193
Lung cancer TMA	Avilabio	DC-Lun01087
Chemicals, peptides, and recombinant proteins
CHX	MedChem Express	HY-12320
MG132	MedChem Express	HY-13259
E64D	MedChem Express	HY-100229
TRITC-phalloidin	Sigma-Aldrich	P1951
CCK8	Dojindo	CK04
DAPI	Beyotime Biotechnology	C1002
Experimental models: Cell lines
H1792	ATCC	CRL-5895
Calu-1	ATCC	HTB-54
H1299	ATCC	CRL-5803
A549	ATCC	CRM-CCL-185
H460	ATCC	HTB-177
H157	ATCC	CRL-5802
BEAS-2B	National Collection of Authenticated Cell Cultures (China)	SCSP-5067
293FT	Invitrogen™	R70007
Oligonucleotides
siRNA targeting sequence: RNF19B #1: CCGGGAGGUGGCCCUGAAUTT	This paper	N/A
siRNA targeting sequence: RNF19B #2: CUCAGCCCCUCUGGCUGUATT	This paper	N/A
Primer: DIRAS3 Forward: GTACCTGCCGACCATTGAAAA Reverse: GGGTTTCCTTCTTGGTGACTG	This paper	N/A
Primer: RAC1 Forward: ATGTCCGTGCAAAGTGGTATC Reverse: CTCGGATCGCTTCGTCAAACA	This paper	N/A
Primer: GAPDH Forward: ACGGATTTGGTCGTATTGGG Reverse: CGCTCCTGGAAGATGGTGAT	This paper	N/A
Recombinant DNA
Plasmid: pcDNA3.1-Flag-DIRAS3	This paper	N/A
Plasmid: pcDNA3.1-Myc-RAC1	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub-K48	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub-K63	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub-K6	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub-K11	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub-K27	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub-K29	This paper	N/A
Plasmid: pcDNA3.1-HA-Ub-K33	This paper	N/A
Plasmid: pcDNA3.1-V5-RNF19B	This paper	N/A
Plasmid: pcDNA3.1-V5-RNF19B-R1	This paper	N/A
Plasmid: pcDNA3.1-V5-RNF19B-R1IBR	This paper	N/A
Plasmid: pcDNA3.1-V5-RNF19B-△R1	This paper	N/A
Plasmid: pcDNA3.1-V5-RNF19B-△R1IBR	This paper	N/A
Plasmid: pcDNA3.1-V5-RNF19B-△R1IBRR2	This paper	N/A
Software and algorithms
SPSS for Windows version 13.0	IBM SPSS	https://www.ibm.com/docs/zh/spss-statistics/
GraphPad Prism 8	GraphPad	https://www.graphpad.com/
Image J	National Institutes of Health	https://imagej.nih.gov/ij/