Figure 3.

Retinal organoids demonstrate transcriptional responsiveness to photostimulation at the miRNA level

(A) Expression of individual members of the miR-183/182/96 cluster 3 h upon photostimulation using white light (100 lx) at different stages of the differentiation process, as determined using RT-qPCR (n = 3). D = dark, L = white light at 100 lx for 3 h.

(B) Principal component analysis (PCA) of miRNA expression profiles in retinal organoids following 1 h and 3 h of photostimulation.

(C) Heatmap illustrates miRNAs that were significantly up- or down-regulated upon photostimulation.

(D) Analysis of miRNA families and clusters that are significantly down-regulated upon photostimulation. Error bars show mean ± SEM (n = 3).

(E) Analysis of miRNA families and clusters that are significantly up-regulated upon photostimulation. Error bars show mean ± SEM (n = 3).

(F) Functional annotation of all significantly up-regulated miRNAs upon photostimulation, as determined using the TAM 2.0 tool.