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. 2023 Jun 28;26(7):107241. doi: 10.1016/j.isci.2023.107241

Figure 5.

Figure 5

Antagonism of beta-3 adrenergic receptors or genetic disruption of Ucp1 blocks increased feeding due to KOR activation

(A) Schematic of experiential paradigm using FED3 devices to measure effects of Saline, U50, or U50 with SR59230A on food intake in wild type and Ucp1−/− mice. Sequence of saline or U50 injections was split among cohort.

(B) Cumulative food eaten in a 24 h window when wild-type mice received saline (black), U50 (red), or U50 with SR59230A (blue) injections at 10:00 a.m. Data are presented as mean ± SEM (n = 15).

(C) Focused view on the 6 h surrounding drug injection with the approximate time span the mice show elevated BAT temperatures in thermal imaging experiments denoted in orange highlighting the sequence of events with BAT activation preceding the increased feeding.

(D) Cumulative food eaten in a 24 h window when Ucp1−/− mice were injected with saline (black) and U50 (red) showing no significant change in feeding in Ucp1−/− mice due to U50.