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. 2023 Jun 28;26(7):107241. doi: 10.1016/j.isci.2023.107241

Figure 6.

Figure 6

High fat diet-induced obesity reversibly disrupts KOR-induced BAT thermogenesis

(A–C) Temperatures recorded with quantitative thermal imaging for BAT, eye, and tail of age-matched lean (black) (9 months old) (n = 8) and HFD fed for 32 weeks (orange) (n = 8) male mice or mice fed HFD for 20 weeks (brown) (6 months old) (n = 8) that received i.p. saline or 5 mg/kg U50. The U50-induced rises in (A) BAT and (B) eye temperatures were not evident in obese fed HFD for 32 weeks mice. However (C), elevations of tail temperatures remained similar in lean and obese mice. Data are presented as mean ± SEM (n = 8 lean and obese).

(D) Schematic of experimental paradigm for transitioning of diet-induced obese mice fed HFD for 32 weeks mice to regular chow and testing the responses to administration of 5 mg/kg U50 (n = 8).

(E) Weights of lean controls, mice fed HFD for 20 and 32 weeks at time of testing. Weights of mice after one and two weeks of normal chow.

(F) Quantified thermal imaging of BAT in diet induced mice while fed HFD and after switching to regular chow for one or two weeks. Mice received 5 mg/kg U50 i.p. (n = 8). (Data are presented as mean ± SEM, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.0002, ∗p = 0.01).