| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological samples | ||
| Stool samples | Healthy and GD volunteers | NA |
| Critical commercial assays | ||
| QIAamp PowerFecal Pro DNA Kit | QIAGEN company in USA | NA |
| Oligonucleotides | ||
| 16S rRNA gene V3-V4 region primer(F 5′-CCTACGGGNGGCWGCAG-3′ and R 5′-GACTACHVGGGTATCTAATCC-3′) | Klindworth et al.62 | NA |
| Deposited data | ||
| Raw and analyzed data | This paper | SRA:PRJNA850658 |
| Software and algorithms | ||
| GraphPad Prism 8 | GraphPad Software Inc. | https://www.graphpad.com/ |
| QIIME v2020.2 | GitHub | http://qiime.org |
| R software | RStudio | https://www.r-project.org/ |
| LEfSe | Galaxy | http://huttenhower.sph.harvard.edu/galaxy |
| STAMP | GNU GPL | http://kiwi.cs.dal.ca/Software/STAMP |
| DADA2 | BiocManager | https://benjjneb.github.io/dada2/dada |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.