Figure 5.

Dynamics and proteomic analysis of mitochondria-LD contacts under metabolic challenge

(A and B) The enriched biotin-labeled proteins were subjected to SDS-PAGE, followed by Western blotting analysis using streptavidin-HRP (A) or Coomassie blue staining (B). See also Figures S6 and S7, Tables S2 and S3 for details.

(C) A volcano plot showing biotinylated proteins under HBSS+OA conditions in comparison to OA conditions. The red dots represent 60 statistically significantly up- or downregulated proteins at mitochondria-LD contact sites upon starvation. See also Table S4 for details.

(D) The subcellular distribution of the 60 proteins with prior annotated localization information in the Uniprot database.