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. 2023 Jun 17;26(7):107159. doi: 10.1016/j.isci.2023.107159

Figure 6.

Figure 6

SQLE accumulates at mitochondria-LD contact sites under metabolic challenge

(A–C) Representative single-plane fluorescent images of HepG2 cells stably expressing SQLE-HA. The cells were incubated in normal conditions (A), OA (B), or HBSS+OA (C) conditions for 16 h and subjected to immunofluorescence labeling. An antibody against HA and CANX (calnexin) was used to detect the SQLE-HA protein and ER, respectively. MitoTracker and LipidTox were used to label mitochondria and LDs, respectively. Scale bar, 5 μm. The lower images are zoom-in views of the boxed regions in the upper images. Scale bar, 2 μm. See also Figures S8 and S9. SQLE-HA, green; mitochondria, magenta; LDs, blue, CANX, gray.

(D) A plot profile of the fluorescence intensity of the white line in (C).

(E and F) Quantification of the percentage of SQLE protein localized at mitochondria-LD contacts (E) or with LDs (F). N = 8 images were analyzed. The experiments shown are a representative of three replicates. Data are presented as the mean ± SEM. NS, no significance. ∗∗p < 0.01, ∗∗∗∗p < 0.0001 (two-tailed t-test).