Biological samples |
|
Sera from individuals presenting with active visceral leishmaniasis confirmed by presence of amastigote forms in bone marrow aspirates stained by Giemsa, and confirmed by isolating parasites in cultures of the bone marrow aspirates in NNN medium. |
Patients hospitalized at the Nathan Portela Institute of Tropical Medicine, Federal University of Piaui or at the University Hospital of the Federal University of Sergipe in 2012-2013; Gardinassi et al.38
|
N/A |
Sera from individuals presenting with active visceral leishmaniasis confirmed by presence of amastigote forms in bone marrow aspirates stained by Giemsa, and confirmed by isolating parasites in cultures of the bone marrow aspirates in NNN medium. |
Patients hospitalized at the Nathan Portela Institute of Tropical Medicine, Federal University of Piaui, 2018; Porcino et al.26
|
N/A |
Sera from individuals presenting with asymptomatic infections or no infection (uninfected endemic controls) |
Contacts (neighbors and household members) of VL patients, 2012-2013; Gardinassi et al.38
|
N/A |
Sera from individuals presenting with asymptomatic infections or no infection (uninfected endemic controls) |
Contacts (neighbors and household members) of VL patients, 2018; Porcino et al.26
|
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
Soluble antigen of L. infantum (SLA) for diagnostic serology and cytokine release assay |
Gardinassi et al.38; Porcino et al.26
|
N/A |
PNGaseF |
Merck, Darmstadt, Germany |
Cat#11365177001 |
|
Critical commercial assays |
|
Kalazar Detect Rapid |
InBIOS International, Seattle, WA, USA |
Cat#INS025 |
OnSite Leishmania IgG/IgM Combo test |
CTK Biotech,San Diego, CA, USA |
Cat#R0122S |
BD Cytometric Bead Array Human Flex Set IP-10 human |
Becton Dickinson Biosciences, USA |
Cat#558280 |
BD Cytometric Bead Array Human Flex Set Anti-MIG human E8 |
Becton Dickinson Biosciences, USA |
Cat#558286 |
Human Soluble Protein Buffer Kit CBA Flex Set |
Becton Dickinson Biosciences, USA |
Cat#558264 |
|
Deposited data |
|
RAW, analyzed and meta data |
https://glycopost.glycosmos.org/ |
GPST000313 |
|
Software and algorithms |
|
Rstudio |
Rstudio Team91
|
https://posit.co/ |
MXM R-package |
Lagani et al.92
|
https://cran.r-project.org/package=MXM |
MassyTools |
Jansen et al.90
|
https://github.com/Tarskin/MassyTools |
|
Other |
|
Hamilton STAR and STARplus robotic system |
Hamilton |
https://www.hamiltoncompany.com/automated-liquid-handling |
Bruker solariX 15T FT-ICR-MS |
Bruker |
https://www.bruker.com/en/products-and-solutions/mass-spectrometry/mrms/solarix.html |