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. 2023 Jun 5;26(7):107021. doi: 10.1016/j.isci.2023.107021
REAGENT or RESOURCE SOURCE IDENTIFIER
Biological samples

Sera from individuals presenting with active visceral leishmaniasis confirmed by presence of amastigote forms in bone marrow aspirates stained by Giemsa, and confirmed by isolating parasites in cultures of the bone marrow aspirates in NNN medium. Patients hospitalized at the Nathan Portela Institute of Tropical Medicine, Federal University of Piaui or at the University Hospital of the Federal University of Sergipe in 2012-2013; Gardinassi et al.38 N/A
Sera from individuals presenting with active visceral leishmaniasis confirmed by presence of amastigote forms in bone marrow aspirates stained by Giemsa, and confirmed by isolating parasites in cultures of the bone marrow aspirates in NNN medium. Patients hospitalized at the Nathan Portela Institute of Tropical Medicine, Federal University of Piaui, 2018; Porcino et al.26 N/A
Sera from individuals presenting with asymptomatic infections or no infection (uninfected endemic controls) Contacts (neighbors and household members) of VL patients, 2012-2013; Gardinassi et al.38 N/A
Sera from individuals presenting with asymptomatic infections or no infection (uninfected endemic controls) Contacts (neighbors and household members) of VL patients, 2018; Porcino et al.26 N/A

Chemicals, peptides, and recombinant proteins

Soluble antigen of L. infantum (SLA) for diagnostic serology and cytokine release assay Gardinassi et al.38; Porcino et al.26 N/A
PNGaseF Merck, Darmstadt, Germany Cat#11365177001

Critical commercial assays

Kalazar Detect Rapid InBIOS International, Seattle, WA, USA Cat#INS025
OnSite Leishmania IgG/IgM Combo test CTK Biotech,San Diego, CA, USA Cat#R0122S
BD Cytometric Bead Array Human Flex Set IP-10 human Becton Dickinson Biosciences, USA Cat#558280
BD Cytometric Bead Array Human Flex Set Anti-MIG human E8 Becton Dickinson Biosciences, USA Cat#558286
Human Soluble Protein Buffer Kit CBA Flex Set Becton Dickinson Biosciences, USA Cat#558264

Deposited data

RAW, analyzed and meta data https://glycopost.glycosmos.org/ GPST000313

Software and algorithms

Rstudio Rstudio Team91 https://posit.co/
MXM R-package Lagani et al.92 https://cran.r-project.org/package=MXM
MassyTools Jansen et al.90 https://github.com/Tarskin/MassyTools

Other

Hamilton STAR and STARplus robotic system Hamilton https://www.hamiltoncompany.com/automated-liquid-handling
Bruker solariX 15T FT-ICR-MS Bruker https://www.bruker.com/en/products-and-solutions/mass-spectrometry/mrms/solarix.html