Figure 5.

Adeno-associated virus serotype 2 (AAV-2)-mediated GFP and NS overexpression in ganglion cells

(A) Schematic representation of the AAV administration experimental timeline. (B) Animal eyes were injected with AAV2 constructs for NS overexpression. GFP alone was used as a control. (C) Quantification of GFP fluorescence represented as the percentage of baseline GFP fluorescence. n = 9, p < 0.0001, paired Student’s t test. (D) Images of retinal sections from mice expressing EGFP (green) after 2 months of viral vector treatment. NeuN (red) was used to stain ganglion cells, and the yellow arrow shows colocalization of NeuN-GFP. A higher magnification of GCL is shown on the right. DAPI (blue) was used to stain nuclei. (E) Fold change in baseline GFP fluorescence. n = 4, p < 0.0001, paired Student’s t test. (F) Images of retinal sections from mice expressing NS (red) after 2 months of viral vector treatment. NeuN (green), colocalization of NeuN-NS (indicated by yellow arrowheads). A higher magnification of GCL is shown on the right. DAPI, blue. (G) Quantification of fluorescence intensity representing fold change in NS (n = 4, p < 0.0001, paired Student’s t test). Scale bars, 50 μm.