Figure 3.

CD8⁺ T cells regulate tumor pyroptosis during CTLA-4 blockade treatment

(A) Representative images of H&E staining and CD8 expression in the control group (Con) and anti-CTLA-4 group of 4MOSC1 tumors (left) and 4MOSC2 tumors (right). Scale bars, 50 μm. (B) CD8⁺ T cell populations in the tumors (left) were measured using flow cytometry, and quantitative analyses were performed (right). (C) Experimental schedule for tumor inoculation and CD8 blockade or CTLA-4 blockade. (D) Tumor volume of 4MOSC1 tumor-bearing mice with different treatments. (E) The production of GSDMD-N and GSDME-N was evaluated using western blotting in different treatment groups for 4MOSC1 tumors (left) and corresponding quantitative analyses (right). Data, mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.