Figure 4.

Synergism of IFN-γ and TNF-α triggers tumor cell pyroptosis

(A) Apoptosis measured using flow cytometry of 4MOSC1 cells 48 h after different treatments. (B) The production of GSDMD-N, GSDME-N, and p-MLKL was evaluated using western blotting in different treatment groups of 4MOSC1 cells. (C) Experimental schedule for tumor inoculation and treatment with IFN-γ blockade or CTLA-4 blockade. (D) Tumor images of 4MOSC1 tumor-bearing mice with different treatments. (E) Tumor volume of 4MOSC1 tumor-bearing mice with different treatments. (F) The production of GSDMD-N and GSDME-N was evaluated using western blotting in different treatment groups for 4MOSC1 tumors (left) and corresponding quantitative analysis (right). Data, mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.