Figure 5.

Synergism of IFN-γ and TNF-α triggers tumor cell pyroptosis via the STAT1/IRF1 Axis

(A) Heatmap depicting the expression levels of genes in 4MOSC1 cells co-treated with TNF-α and IFN-γ for 48 h relative to their expression in untreated 4MOSC1 cells. (B) The expression of p-Stat1, IRF-1, Cle-caspase-8, Cle-caspase-3, and p-MLKL in 4MOSC1 cells and the production of GSDMD-N and GSDME-N in 4MOSC1 cells were evaluated using western blotting in different treatment groups (left) and corresponding quantitative analyses (right). (C) Representative bright-field microscopy images of 4MOSC1 cells with different treatments. The arrows indicate pyroptotic cells. Scale bars, 20 μm. (D) Schematic overview of TNF-α- and IFN-γ-induced tumor cell pyroptosis and inflammatory factor and chemokine release. Data, mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.