Figure 6.

Synergism of IFN-γ and TNF-α triggers pyroptosis in human HNSCC cells and tissues

(A) The expression of GSDMD and GSDME was evaluated using western blotting in Cal27, SCC4, SCC9, and SCC25 human HNSCC cell lines (left) and corresponding quantitative analyses (right). (B) Schematic overview of SCC9 cells and fresh HNSCC tissue stimulated with the cytokines IFN-γ and TNF-α. (C) The production of GSDMD-N and GSDME-N was evaluated using western blotting in different treatment groups of SCC9 cells (left) and corresponding quantitative analyses (right). (D) Representative bright-field microscopy images of SCC9 cells with different treatments. The arrows indicate pyroptotic cells. Scale bars, 20 μm. (E) Quantitative analyses of GSDMD-N and GSDME-N production in different treatment groups of HNSCC tumors as assessed using western blotting. Data, mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.