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. 2023 Mar 24;31(7):1920–1937. doi: 10.1016/j.ymthe.2023.03.024

Figure 2.

Figure 2

Overview of different CRISPRa and CRISPRi platforms

Transcriptional modulation is mediated by localization of transcriptional activators or inhibitors to the region around the transcriptional start site (TSS). For CRISPRa, multiple activators can be recruited using either the sgRNA scaffold (SAM) or an array of epitopes fused to dCas (SunTag). For CRISPRi, steric hindrance can interfere with transcription using a dCas protein alone, but transcriptional inhibition is improved using a fused transcriptional repression domain.