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. 2023 Mar 24;31(7):1920–1937. doi: 10.1016/j.ymthe.2023.03.024

Figure 3.

Figure 3

Delivery modalities for CRISPR-Cas-based transcriptional modulators

Retro/lentiviral, AAV, and plasmid vectors encode both dCas-effector and sgRNA, and whereas lentiviral vectors integrate these expression cassettes into the genome, AAV and plasmid vectors are transcribed episomally. RNA delivery relies on in-vitro-transcribed RNA encoding dCas-effector and synthetic sgRNA delivery. Some dCas-effector proteins can be generated as recombinant protein and precomplexed into an RNP complex using synthetic sgRNAs prior to delivery.