Table 2.
An overview of CRISPRa and CRISPRi genetic screens
| Reference | CRISPRa/i construct | Target cells | Purpose |
|---|---|---|---|
| Le Sage et al.28 | dSpCas-KRAB, dSpCas9-VP64 (SAM) | A375 melanoma cells | uncovering gene networks driving drug resistance |
| Boettcher et al.61 | dSpCas9-10xGCN4 (SunTag) + scFv-GCN4-sfGFP-VP64 | K562 cells | analyzing the quantitative effects of activation and KO to reconstruct directional gene dependency networks for K562 cells |
| Liu et al.60 | dSpCas9-10xGCN4 (SunTag) + scFv-GCN4-sfGFP-VP64 | mouse embryonic stem cells | identification of factors that drive neuronal fate and reprogramming |
| Yang et al.69 | dSpCas9-VP64 (SAM) | primed human epiblast stem cells | identification of novel factors related to cellular reprogramming |
| Strezoska et al.52 | dSpCas9-VPR | U2OS cells | identification of regulators of IL-6 cytokine secretion |
| Black et al.45 | VP64-dSpCas9-VP64, dSaCas9-KRAB | human iPSCs | identification of master regulators and cofactors related to human neuronal cell fate specification |
| Tian et al.27 | dSpCas9-VPH, dSpCas9-KRAB | human iPSCs | lysosomal failure linked to ferroptosis in human neurons |
| Li et al.66 | dSpCas9-VP64 (SAM) | human HCC cell lines | identification of regulators of sorafenib resistance |
| Zhu et al.49 | dSpCas9-VP64 | HEK293T, Huh7 | identification of candidate receptors for SARS-CoV-2 entry |
| Dräger et al.29 | dSpCas9-KRAB, | human iPSCs | uncovering regulators of microglial states, enabling their functional characterization and therapeutic targeting |
| Thompson et al.50 | dSpCas9-VP64 | keratinocytes | identification of genes and pathways that increase type VII collagen (C7) expression. |
| Danziger et al.63 | dSpCas9-10xGCN4 (SunTag) + scFv-GCN4-sfGFP-VP64 | A549 | identification of interferon-stimulated genes, SARS-CoV-2 restriction factor identification |
| Unlu et al.55 | dSpCas9-VPR | Jurkat, HepG2, HEK293T, MDA-ME-231, PaTu-8988t | identification of SLCO2B1 as a heme transporter that enhances cellular iron availability |
| Siepe et al.64 | dSpCas9-10xGCN4 (SunTag) + scFv-GCN4-sfGFP-VP64 | K562 | identification of orphan ligand-receptor relationships |
| Leng et al.39 | dSpCas9-KRAB | HiPSC-derived astrocytes “iAstrocytes” | identifying cellular pathways controlling inflammatory astrocyte reactivity induced by IL-1α+TNF0C1q |
| Schmidt et al.31 | dSpCas9-VP64 (SAM), dSpCas9-KRAB | primary human T cells | identifying regulators of cytokine production |
| Jung et al.30 | dSpCas9-10xGCN4 (SunTag) + scFv-GCN4-sfGFP-VP64, dSpCas9-KRAB | 32Dcl2 | identifying genetic networks in HDAC-inhibitor-resistant cells |
| Zhang et al.122 | dSpCas9-VP64 (SAM) | HEPG2 | in vivo study to screen for drivers of hepatocellular carcinoma growth and metastasis |