Figure 3.

Optical coherence tomography and fundus angiography

Quantitation and representative optical coherence tomography images demonstrate that increased expression of BACE1 at the low or high dose 6 months following subretinal injection maintains normal retina structure in contrast to SOD2 KD eyes alone, which suffered severe abnormalities and thinning in the retina - mean ± SEM (A–C). Quantification demonstrated this protective effect was significant in eyes receiving the low dose at 1 month following AAV-BACE1 delivery (A) while some pathological changes in the retinal and RPE layer were observed with high-dose BACE1 (A). By the 6-month time point, no retinal pathology was apparent at which time the protective effects were significant both for low and high dose of AAV-BACE1 delivery (B). Fundus angiography demonstrated improved retinal morphology and less fluorescence leakage in SOD knockout eyes after receiving AAV-BACE1 injection (D). n = 5 for each study group.