Figure 7.

AAV1-BACE1 inhibited oxidative stress in SOD2 KD mice

Immunostaining for the oxidative stress marker 4-hydroxynonenal (4-HNE) in SOD2 KD mice at 6 months following AAV-BACE1 injection showed a dramatic increase in 4-HNE staining (red) in the neural retina and RPE compared with normal untreated eyes. Staining was primarily located to the RGC layer, inner and outer nuclear layer, and RPE. Retina vasculature was stained with agglutinin in green. By contrast, overexpression of BACE1 at either the low or high doses significantly reduced 4-HNE levels (p < 0.01, mean ± SEM, n = 5).