Figure 6.

Human and murine anti-AAV9 Fabs bind the four topological regions of the AAV capsid

(A) Top row: mouse Fabs binding to the 3-fold, 2/5-fold wall, 2-fold, and 5-fold regions are shown. In the absence of a density map for PAV9.1,¹⁷ a surface map from a pseudoatomic model was generated. The maps are radially colored according to distance to the center of the capsid (blue to red, as in Figure 6). Lower row: a selection of the human mAbs that bind regions equivalent to the murine mAbs. For the ADK9 and Fab2-4 density maps, the location of the 3-fold protrusions (3fp) are indicated. (B) The atomic model of AAV9-AAVR (PDB: 7WJX) was fitted into the density maps to determine whether the human Fabs overlap with AAVR (blue ribbon).