Table 1.
Frequency of smB cells expressing AAV9-reactive antibody
PBMC source | Days post-antigen stimulus | Wells seeded with smB cells | IgG+ wells | IgG+ wells, % | AAV9 reactive clones | Anti-AAV9 reactive smB clones (%) |
---|---|---|---|---|---|---|
Patient 1 | 324 | 2,464 | 714 | 29 | 39 | 5.5 |
Patient 2 | 151 | 1,232 | 221 | 18 | 22 | 9.9 |
Patient 3 | 259 | 616 | 363 | 59 | 8 | 2.2 |
Mother of patient 5 | unknown | 9,856 | 7,491 | 76 | 1 | <0.1 |