Figure 1.

Reporter gene expression in nonhuman primates following subretinal injection

(A) Retinal section across injection bleb showing GFP expression (green) within the bleb. Blue is DAPI nuclear stain. Size bar, 100 μm. (B) Representative sections from blebs of the four dose groups (B1–B4). In each case, GFP expression (using anti-GFP antibody) is shown in green in the left panel, and GFP expression merged with DAPI (blue) is shown in the right panel. Doses (vector genomes) are indicated above each panel, and animal number, eye, and bleb imaged are indicated beside each panel. A dose effect is evident. Size bar, 50 μm. Key: INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; IS, inner segment; OS, outer segment. (C) Comparative number of GFP expressing outer nuclear cell nuclei per unit length for each dose group, illustrating the dose effect. The color key indicates the individual animals.