FIG. 4.
Sequence and sequence analysis of the Sau3AI (8.10)-BclI (14.8) SLP1 region upstream of Popimp. (A) The sequence of the first 200 bp of the Sau3AI (8.10)-Sau3AI (8.66) region and of the 200 bp prior to the spdB1 gene (as determined previously [15]) are shown and are separated by dashed lines. Recognition sequences sites for the restriction enzymes shown are underlined, as are sequences which function as putative ribosome-binding sites (RBS). Translation of the different ORFs is shown above the nucleotide sequence. The arrows originating at G, 5′ of impA, and at T, 5′ of spdB1, indicate the Pimp and Popimp transcription start sites, respectively. Dashed arrows indicate inverted repeats. Direct repeats (DR) are underlined. The −35 and −10 hexamers are indicated by heavy lines above the sequence. (B) Complete sequence Sau3AI (8.10)-BclI (14.8). The ORFs are indicated by arrows. Putative transmembrane helices of the putative protein are indicated by TM. The restriction sites are shown. Numbers indicate nucleotide positions of the start and stop sites of the ORFs in the sequence.