FIG. 5.
Tn5 mutational analysis of the ORFs located in the Sau3AI (8.10)-BclI (14.8) SLP1 region upstream of Popimp. Arrows represent the orientation of ORFs. The small triangles and dashed lines represent locations of the Tn5 insertions. The pock sizes formed by plasmids are represented by +++, ++, +, and −, for normal, small, and tiny pocks and no pocks, respectively. Plasmid transfer efficiency was calculated as described in reference 20. The ORFs are indicated by arrows. Plasmids and the Streptomyces strains harboring them are shown.