Parameters affecting CAR efficacy
To determine the effect of the binder biophysical properties to the CAR efficacy, we calculated two-dimensional correlation plots of Ig domain (A), association rate (Ka) (B), dissociation rate (Kd) (C), affinity (KD) (D), binder stability (°C) (E), and aggregation (F) to the CAR efficacy. For the CAR efficacy, we introduced the cytotoxicity of the novel aCD22 binders against SupT1 CD22High (blue) or CD22Mid (red) in 1:4 ratio. The r values represent Pearson correlation coefficients (n = 8–12).