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. 2023 May 16;31(7):2206–2219. doi: 10.1016/j.ymthe.2023.05.011

Figure 1.

Figure 1

Mouse brain-implanted with human neural progenitor cells retain proliferation and stemness status

(A) Cartoon of lentiviral vector (Addgene #73022) to constitutively express copGFP and NanoLuciferase (NanoLuc) in iPSC-derived hNPCs (left). In vivo bioluminescence imaging in the brains of mice emitted by implanted hNPCs expressing copGFP and NanoLuciferase (NanoLuc). Image acquired with IVIS Living Image software (right). (B–D) Immunohistochemistry on brain sections from hNPC (delineated with dotted line) implanted mice evaluated with (B) stemness marker nestin (red) as well as copGFP (green), DAPI (blue), and merged image. (C) Stemness marker Pax6 (purple), copGFP (green), DAPI (blue), and merged image. (D) Proliferation marker Ki67 (red), copGFP (green), DAPI (blue), and merged image. Analysis was performed 21 days after injecting both patient and healthy control hNPCs into mouse brains. In all panels, an arrow indicates the injection site and scale bars of 100 μm are indicated. Immunofluorescence was imaged with a Keyence BZ-X810 fluorescent microscope. (n = 4 per staining group with representative images shown: B and C from XDP and D from CON).