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. 2023 Apr 17;31(7):2105–2119. doi: 10.1016/j.ymthe.2023.04.008

Figure 3.

Figure 3

HFRT promotes intratumoral infiltration of MDSCs and T cells from peripheral blood and induces a suppressive phenotype of MDSCs

(A) Experimental scheme. (B and C) Bar plots showing fractions of GFP+ MDSCs and T cells in tumors by flow cytometry on day 5 after radiation initiation. Representative immunofluorescence images show GFP+ MDSCs or T cells in tumors in the indicated groups (n = 3; scale bar: 50 μm). (D) Representative multicolor immunofluorescence images of CT26 tumor (n = 3; scale bar: 100 μm; MDSCs: red arrowheads, T cells: white arrowheads). (E) KEGG enrichment analysis of differentially expressed genes (DEGs) in irradiated CT26 tumors compared with the unirradiated counterpart. (F) GSEA of the Jak-STAT signaling pathway in MDSCs from CT26 tumor. (G) Heatmap of DEGs that encoding genes related to proliferation and activation of MDSCs. (H) GSEA of PD-L1 expression and the PD-1 checkpoint pathway in MDSCs from CT26 tumor. (I) Bar plots showing fractions of PD-L1+ MDSCs in tumors. Results are presented as mean ± SEM, unpaired Student’s t test. (J) Treatment scheme and tumor volume were plotted starting from the day before the initial dose of HFRT (n = 7; results are presented as mean ± SEM; mixed-effects analysis). ∗p < 0.05 and ∗∗p < 0.01; NS, p > 0.05.