Figure 4.

HFRT-mediated MDSC recruitment in a chemokine-chemokine receptor-dependent manner

(A) GSEA of chemokine signaling pathways in MDSCs isolated from the tumor, spleen, and bone marrow (BM) of mice bearing CT26 tumor on day 7 after HFRT. (B) Heatmap of genes encoding chemokine receptors in MDSCs from different tissues. (C) Heatmap of DEGs that encoding chemokines of MDSCs in the tumor bulk. (D and E) Representative cytokine arrays for the proteins of serum and tumors; the boxes indicate the chemokines with significant increases (n = 3). Logarithm of fold change (log₂RT/control) ratios for differentially expressed chemokines between the HFRT group and control group in serum and tumor. (F) Fluor cytometric analysis of CCR2⁺ MDSCs, CCR5⁺ MDSCs, and CXCR2⁺ MDSC accumulation in tumors and the quantification are presented. Results are presented as mean ± SEM (n = 5). ∗p < 0.05 and ∗∗p <0.01; NS, p > 0.05 (unpaired Student’s t test). (G) Heatmap of DEGs in tumor bulk encoding chemokines that are chemotactic for T cells. (H) Logarithm of fold change (log₂RT/Control) ratios for seven differentially expressed T cell-related chemokines in serum and tumor.