Table 2.
Effect of OD, dipping duration, and Silwet L-77 on the average number of seeds and plants resistant to kanamycin and transformation efficiency of T1 C. sulphureus Cav.
|
Treatments |
Results |
||||||
|---|---|---|---|---|---|---|---|
| Experiment | Agrobacterium suspension | Silwet L-77 (%) | Duration (s) | Number of seeds | Number of seedlings | Resistant to Kanamycin (%) | Transformation efficiency (%) |
| Control | 0 | 0 | 14.5 ± 2.7a | 0 ± 0d | 0 ± 0c | 0 ± 0b | |
| I | 0 | 30 | 3.40 ± 1.5d | 0.8 ± 1.0c | 23.06 ± 23.6b | 0 ± 0b | |
| OD600 = 1.5 | 0 | 60 | 10.2 ± 3.2bc | 4.6 ± 1.6a | 45.32 ± 11.7a | 0 ± 0b | |
| 0 | 90 | 2.00 ± 1.7d | 0.9 ± 1.1c | 26.00 ± 28.6b | 0 ± 0b | ||
| 0 | 30 | 12.3 ± 2.0a | 1.8 ± 1.3bc | 14.63 ± 11.9b | 8.26 ± 6.5a | ||
| OD600 = 0.8 | 0 | 60 | 9.00 ± 2.7c | 2.2 ± 1.4b | 24.97 ± 15.8b | 2.26 ± 4.9b | |
| 0 | 90 | 11.1 ± 2.4ab | 1.3 ± 1.4bc | 11.71 ± 10.5b | 0 ± 0b | ||
| II | OD600 = 0.8 + 5% sucrose | 0 | 30 | 15.4 ± 4.0a | 4.2 ± 3.2a | 25.35 ± 19.2a | 9.74 ± 2.0b |
| 0.05 | 30 | 12.9 ± 2.5a | 4.2 ± 3.6a | 32.52 ± 29.2a | 9.38 ± 2.3b | ||
| 0.1 | 30 | 13.3 ± 4.8a | 4.5 ± 3.0a | 35.11 ± 19.4a | 12.78 ± 1.5a | ||
Note that the number followed by the same letters is not significantly different based on the DMRT test; α < 0.05.