Table 1.
Primer sequences used in qRT-PCR
| Primer name | Primer sequence | Observations |
|---|---|---|
| Forward Cre PP5 | CGCGGTCTGGCAGTAAAAAC | |
| Reverse Cre PP5 | GTTCGAACGCTAGAGCCTGT | |
| Forward 5′ Cre PP10 | CGGTCGATGCAACGAGTGAT | |
| Reverse 5′ Cre PP10 | CAGGTATGCTCAGAAAACGCC | |
| Forward 3′ Cre PP7 | ACCAGCCAGCTATCAACTCG | |
| Reverse 3′ Cre PP7 | ACCATTGCCCCTGTTTCACT | |
| Forward h2bFirefLy_PP4 | GGAGAGCAACTGCATAAGGC | |
| Reverse h2bFirefLy_PP4 | CACTACGGTAGGCTGCGAAA | |
| Forward Nanoluc_PP2 | AAGGATTGTCCTGAGCGGTG | |
| Reverse Nanoluc_PP2 | AACACGGCGATGCCTTCATA | forward primer for FLEx unfloxed sequence (FLExOFF) reverse primer for FLEx floxed sequence (FLExON) |
| Forward EF1A_PP2 | GGGGAGGGGTTTTATGCGAT | forward primer for FLEx floxed sequence (FLExON) |
| Reverse EF1A_PP2 | CGCTATGTGGATACGCTGCT | |
| Forward WPRE | CGCTATGTGGATACGCTGCT | |
| Reverse WPRE | GTTGCGTCAGCAAACACAGT | reverse primer for FLEx unfloxed sequence (FLExOFF) |
| Forward hHPRT | TTGCTTTCCTTGGTCAGGCA | |
| Reverse hHPRT | ATCCAACACTTCGTGGGGTC | |
| Forward mHPRT | CATCCTCCTCAGACCGCTTT | |
| Reverse mHPRT | TCATCGCTAATCACGACGCT | |
| Forward hGAPDH | CCCCGGTTTCTATAAATTGAGCC | |
| Reverse hGAPDH | TGGCTCGGCTGGCGAC | |
| Forward mGAPDH | TGGAGAAACCTGCCAAGTATGA | |
| Reverse mGAPDH | GGTCCTCAGTGTAGCCCAAG | |
| Forward Ai9 CAG | GCAACGTGCTGGTTATTGTG | forward primer for Ai9 sequence |
| Reverse UNFLOXED | TGCAAGCTTTCATTTATTCATCGC | reverse primer for stop/poly(A) region between loxP regions |
| Reverse Ai9_U1140_ | TTTGATGACCTCCTCGCCCT | reverse primer for tdTomato sequence |