Figure 3.

Therapeutic effects of AAV-XBP1s administration into the hippocampus of AD mice

WT and 5xFAD mice were injected with either AAV-XBP1s or AAV-Mock at 1.5 months of age and sacrificed after 8–9 months. Learning curves using the Morris water maze (A) and Barnes maze (BM) (B) behavioral tests were measured. Long-term memory was measured at 12 days post-training of BM paradigm (C). Percentage of alternation of behavioral paradigm Y maze (D). Data values are expressed as mean ± SEM. WT^AVV-Mock (n = 7–10), WT^AVV-XBP1s (n = 7–10), 5xFAD^AVV-Mock (n = 7–10), and 5xFAD^AVV-XBP1s (n = 7–10). Data from (C) and (D) were analyzed using one-way ANOVA followed by Tukey’s multiple-comparison post-test. Data from (A) and (B) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparison post-test. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.