Figure 4.

AAV-XBP1s administration into the hippocampus of 5xFAD mice improves synaptic function

(A) Dendritic spine density of pyramidal neurons in the CA1 region was measured in indicated experimental groups. Left panel: representative images of dendritic spines, and the quantification (right panel). Scale bar: 100 μm. (B) Time course of the TBS-induced LTP in slices prepared from all experimental groups and (C) quantification of LTP using hippocampal slices. (D) Representative images of amyloid deposits in the hippocampus (left panel), and quantification of amyloid β load, and the number of plaques of injected animals (right panel). Scale bar: 100 μm. Data values are expressed as mean ± SEM. WT^AVV-Mock (n = 7–10), WT^AVV-XBP1s (n = 7–10), 5xFAD^AVV-Mock (n = 7–10), and 5xFAD^AVV-XBP1s (n = 7–10). Data were analyzed using one-way ANOVA followed by Tukey’s multiple-comparison post-test. Data from (B) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparison post-test. ∗p < 0.05 and ∗∗∗p < 0.001.