Figure 5.

Comparative proteomics analysis of hippocampal tissue of AD mice overexpressing XBP1s

(A) The significance score in the heatmap represents a combination of the statistical significance and the direction of change (see materials and methods). The values at the top represent Spearman’s correlation coefficient between the groups. (B) Overlap of the biological processes regulated in opposite directions in WT vs. 5xFAD and 5xFAD vs. Tg^XBP1s/5xFAD mice. Top 10 enriched overlapping processes are displayed in the dashed boxes. p values at the bottom represent the statistical significance of the overlap. (C) Cfl1 western blot (upper panel) and the respective quantification of hippocampal lysate (lower panel) from WT, Tg^XBP1s, 5xFAD, and Tg^XBP1s/5xFAD mice, using α-tubulin as a housekeeping control. (D) Proteomic changes in the hippocampus of Alzheimer’s disease patients and humans at different ages for the top proteins restored by XBP1s overexpression. At the top, we show the mean fold changes for the candidate proteins in each comparison group. In parentheses, we indicate the number of proteins measured in each dataset. The dot plot below displays the quantile normalized fold change values of the proteins in each dataset (dot color). p values at the bottom indicate the statistical significance from a t test with population mean equal zero.