Figure 1.

Differentiation and growth of hCSs

(A) Schematic of differentiation of iPSCs to hCSs including the specific time points and factors used for differentiation and readouts.

(B) Representative bright-field images of male control and PSEN1 L435F hCSs at each time point from 14 to 365 DIV. Scale bar, 500 μm (for all images).

(C) Quantification of area of male hCSs at each time point. n = 10–15 hCSs from 2 to 3 independent replicates.

(D) Circularity measure of male hCSs at each time point. Data are presented as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 from unpaired t tests per time point.