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. 2023 Jun 22;18(7):1516–1533. doi: 10.1016/j.stemcr.2023.05.018

Figure 2.

Figure 2

PSEN1 L435F increases PAX6+ neural progenitors and VZs at day 30

(A) Representative IHC images of male control and PSEN1 L435F hCSs stained for PAX6 and DAPI.

(B and C) (B) Quantification of PAX6 at 30 DIV and (C) quantification of VZs at 30 DIV in male hCSs; VZs defined as a circular PAX6+ structure. N = 10–12 hCSs from 4 individual replicates, including outliers. Scale bars, 50 μm.

(D) Representative IHC images of male hCSs stained for KI67 and DAPI at 30 DIV.

(E and F) (E) Quantification of KI67 and (F) BrdU in male hCSs at 30 DIV. N = 9–12 hCSs from 3 to 4 independent replicates. Data are presented as mean ± SEM. p value from unpaired t tests and Cohen’s d value for effect size are shown.