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Effects of aphidicolin and preincubation temperature on DNA polymerizing activity. The standard assay mixture including fraction I (F1) or II (F2) was incubated at 70°C for 5 min in the presence of the indicated levels of aphidicolin (a), or the enzyme fractions were incubated at the indicated temperatures for 30 min before the activities were measured under the conditions described above (b). P. furiosus (Pfu) Pol I served as a control.
