FIG. 3.
Activity gel analysis of DNA polymerizing activities in fractionated A. pernix cell extracts, fraction I and fraction II, and recombinant Pol I and Pol II. DNA polymerizing activity was detected by in situ incorporation of [α-32P]dCTP as described in Materials and Methods. Proteins in fraction I (F1), fraction II (F2), purified Pol I (PI), and Pol II (PII) were separated by SDS-PAGE, and 32P incorporated into DNA strands in the gel was detected by autoradiography. The molecular masses indicated on the left were derived from a size standard marker (Bio-Rad, Hercules, Calif.).