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Effects of aphidicolin and preincubation temperature on DNA polymerizing activity of A. pernix Pol I and Pol II. Aliquots of purified A. pernix (Ape) Pol I or Pol II were incubated at 70°C for 5 min in the presence of the indicated levels of aphidicolin (a), or the enzyme fractions were incubated at indicated temperatures for 30 min before the activities were measured under the conditions described above (b). P. furiosus (Pfu) Pol I served as a control.
