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. 2023 Jun 22;18(7):1500–1515. doi: 10.1016/j.stemcr.2023.05.020

Figure 6.

Figure 6

PHOX2B-PARM interrupts the pattern specification of PHOX2B+ neurons in HCOs

(A) Immunostaining showing the nuclear localization of PHOX2B proteins in the day-60 control and PHOX2B-7Ala mutant HCOs.

(B) GRN activity of PHOX2B in the control and mutant HCOs.

(C) Violin plots showing the expression of PHOX2B hindbrain targets in the control and mutant CNs. ∗∗p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

(D) Scatterplot showing the putative PHOX2B-7Ala activated (red) and repressed (blue) targeted genes.

(E) Gene Ontology (GO) enrichment analyses of PHOX2B activated and repressed targeted genes.

(F) Overall pathway scores of embryonic organ morphogenesis and pattern specification. ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001; ns, non-significant.

(G) Violin plots showing the expression of HOX genes in the control and mutant CNs. ∗∗∗∗p < 0.0001.

(H) Violin plots showing the expression of Hedgehog pathway genes in the control and mutant CNs. ∗∗p < 0.05 and ∗∗∗∗p < 0.0001.

(I) Immunostaining of PHOX2B and markers of forebrain, midbrain, and hindbrain (TBR1, FOXA2, and ISL1) in the day-60 PHOX2B-7Ala HCOs.

(J) Immunostaining of PHOX2B and OTX2 in the day-60 control and PHOX2B-7Ala HCOs. An enlargement of a square region in the image is shown in the (I) bottom and (J) right panel.