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. 2023 Jun 15;18(7):1451–1467. doi: 10.1016/j.stemcr.2023.05.012

Figure 2.

Figure 2

Ala-Gln supplementation increases the number and proliferation rate of ISCs in vivo

(A) Intestinal stem cells labeled with OLFM4 (green) in the section of the proximal jejunum. Scale bars, 40 μm.

(B) The number of ISC were quantified (n = 6 mice, means ± SEM; ∗p < 0.05, ∗∗p < 0.01; one-way ANOVA, results of 3 independent experiments).

(C) Intestinal stem cells were labeled with LGR5 (red) in the section of the proximal jejunum. Scale bar, 50 μm.

(D) Representative immunofluorescence images of ISC stained with OLFM4 (red) and DAPI (blue) are shown in the section of the proximal jejunum. Scale bars, 50 μm.

(E) Quantification of integrate optical density (n = 6 mice, means ± SEM; ∗p < 0.05, ∗∗p < 0.01; one-way ANOVA, results of 3 independent experiments).

(F) Representative images of crypt base columnar cells of immunofluorescence stained with KI67 (red) and DAPI (blue). Scale bars, 50 μm.

(G) Quantification of KI67-positive crypt base columnar cells (n = 6 mice, means ± SEM; ∗p < 0.05, ∗∗p < 0.01; one-way ANOVA, results of 3 independent experiments).