Figure 1.

Lack of Serine Racemase leads to defects in adult SVZ neurogenesis and proliferation in vivo

(A) Expression of SR in WT and SR^−/− mice SVZ lysates.

(B) WT and SR^−/− SVZ lateral ventricle showing BrdU incorporation (red) and GFAP expression (green). Scale bar, 100 μm.

(C) Quantitative estimation of BrdU+ cells.

(D–H) BrdU incorporation in NSC from WT and SR^−/− SVZ. Inset shows incorporation of BrdU in NSCs (red). Expression of (E) β-III Tubulin and (F) NEUROD1 in NSCs from WT and SR^−/− mice after differentiation. Relative mRNA levels of (G) Sox2 and (H) Mash1 in olfactory bulb of WT and SR^−/− mice. Error bars represent SD. Data are representative of three independent experiments and tissues pooled from n = 5–6 mice/experiment.

(I) Expression of DLX2 and OLIG 2 in NSC lysates.

(J) Mouse olfactory behavior test in age-matched WT and SR^−/− mice. N = number of mice.