Figure 3.

Serine Racemase alters L-serine and de novo fatty acid synthesis in the SVZ of nestin-cre+ mice

(A) Expression of PGDH (green) and PSPH (red) in the SVZ of SR ^flox/flox and nestin-cre+ mice. Scale bar, 20 μm.

(B) Relative mRNA levels of Pgdh and Psph. Data are representative of three independent experiments. Tissues were pooled from n = 5–8 mice.

(C) Expression of PSPH and PGDH in SVZ.

(D) Expression of mouse FASN in SVZ. Scale bar, 20 μm.

(E) Expression of PSPH and PGDH in NSC lysates.

(F) Mouse FASN expression in whole brain homogenates. ^∗p = 0.047 relative to SR^flox/flox.

(G) Expression of SPOT14 in SVZ.

(H) Expression of mouse FASN in SVZ. ^∗p = 0.05 relative to SR ^flox/flox.

(I) mRNA levels of SPOT14 regulator Mig12 in SVZ of WT and SR^−/− mice.

(J) Estimation of malonyl-CoA in SVZ by ELISA. ^∗p = 0.054 relative to SR ^flox/flox. Error bars are SD. Data are representative of three independent experiments. Tissues were pooled from n = 5–8 mice per experiment.