Figure 6.

L- and D-serine rescue defects in SVZ neurogenesis and proliferation in vitro

(A) BrdU incorporation in WT and SR^−/− NSCs treated with 10 μM BrdU for 18 h at 37°C.

(B) BrdU incorporation in cells treated with 0.6 mM L-serine.

(C) BrdU incorporation in cells treated with 0.1 mM D-serine.

(D) Expression of SOX2 in control (top) and NSCs treated with equimolar mixture of L- and D-serine (100 μM) (lower panel).

(E) Expression of NEUN in differentiating neurons in control (top) and mixture of L- and D-serine (bottom). Scale bar, 100 μm.

(F) Number of BrdU-positive cells per high-powered field in NSCs from WT and SR^−/− mice SVZ. An average of 4–5 fields were counted. Error bars represent SD.

(G) Expression of AMPK-α in WT and SR^−/− NSCs.